For many years, aptamers were developed using one of several versions of the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process. X-Aptamers are the next step in the evolution of aptamer technology. They are synthetic affinity reagents that incorporate natural and chemically-modified DNA or RNA nucleotides. Common modifications include amino acid functional groups, as well as small molecules in virtually any combination.
AM Biotech uses a proprietary bead-based technology to select X-Aptamers. Our single round discovery process has significant advantages relative to SELEX. AM Biotech’s selection eliminates the repeated rounds of PCR amplification, unlike SELEX who's many rounds lead to PCR bias – which disfavors recovery of the most structurally stable aptamers.
Improve Existing Aptamers & siRNA
AM Biotech uses chemical modifications to improve existing aptamer and siRNA performance. DNA or RNA aptamer binding affinity can improve dramatically without adversely affecting specificity by using just one or two modified nucleotides in a distinct sequence location.